Optimized oxidoreductases for medium and large scale industrial biotransformations
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Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
Phone: 34 918373112
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publications
Total records: 126
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[ 2014 ] Levasseur A, Lomascolo A, Chabrol O, Ruiz-Dueñas FJ, [...] , Martínez AT, [...] , Record E The genome of the white-rot fungus Pycnoporus cinnabarinus: a basidiomycete model with a versatile arsenal for lignocellulosic biomass breakdown BMC Genomics, 15: 486
[ 2014 ] Linde D, Coscolín C, Liers C, Hofrichter M, Martínez AT, Ruiz-Dueñas FJ Heterologous expression and physicochemical characterization of a fungal dye-decolorizing peroxidase from Auricularia auricula-judae Protein Expr. Purif., 103: 28-37
[ 2014 ] Macellaro G, Baratto MC, Piscitelli A, Pezzella C, Fabrizi de Biani F, Palmese A, Piumi F, Record E, Basosi R, Sannia G Effective mutations in a high redox potential laccase from Pleurotus ostreatus Appl. Microbiol. Biotechnol., doi: 10.1007/s00253-013-5491-8
[ 2014 ] Macellaro G, Pezzella C, Cicatiello P, Sannia G, Piscitelli A Fungal Laccases Degradation of Endocrine Disrupting Compounds BioMed Research International, doi: 10.1155/2014/614038
[ 2014 ] Martínez AT, Ruiz-Dueñas FJ, Gutiérrez A, del Río JC, Alcalde M, Liers C, Ullrich R, Hofrichter M, Scheibner K, Kalum L, Vind J, Lund H Search, engineering, and applications of new oxidative biocatalysts Biofuels, Bioprod. Bioref., 8: 819-835
[ 2014 ] Molina-Espeja P, García-Ruiz E, González-Pérez D, Ullrich R, Hofrichter M, Alcalde M Directed evolution of Unspecific Peroxygenase from Agrocybe aegerita Appl. Environ. Microbiol., 80: 3496-3507
year2013
Increasing the Productivity of Peroxidase-Catalyzed Oxyfunctionalization: A Case Study on the Potential of Two-Liquid-Phase Systems
Churakova E, Arends IWCE, Hollmann F
ChemCatChem, 5: 565-568

Biocatalysis, which occurs mostly in aqueous reaction media, is generally incompatible with the hydrophobic nature of many reactants. This may be overcome by using the two-liquid-phase system (2 LPS) approach. We demonstrate the beneficial application of the 2 LPS approach for the photobiocatalytic oxyfunctionalization of thioanisole. Compared to aqueous reaction conditions, the 2 LPS approach not only allowed for higher substrate loadings (without the use of additional solvents) but also increased the productivity and robustness of the overall reaction.

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón