Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2015 ]
Poraj-Kobielska M, Peter S, Leonhardt S, Ullrich R, Scheibner K, Hofrichter M Immobilization of unspecific peroxygenases (EC 1.11.2.1) in PVA/PEG gel and hollow fiber modules
Biochem. Eng. J., 98: 144-150
[ 2015 ]
Rico A, Rencoret J, del Río JC, Martínez AT, Gutiérrez A In-Depth 2D NMR Study of Lignin Modification During Pretreatment of Eucalyptus Wood with Laccase and Mediators
Bioenerg. Res., 8: 211-230
[ 2015 ]
Saez-Jimenez V, Acebes S, Guallar V, Martínez AT, Ruiz-Dueñas FJ Improving the oxidative stability of a high redox potential fungal peroxidase by rational design
PlosOne, 10-4
[ 2015 ]
Saez-Jimenez V, Baratto MC, Pogni R, Rencoret J, Gutiérrez A, Santos JI, Martínez AT, Ruiz-Dueñas FJ Demonstration of Lignin-to-Peroxidase Direct Electron Transfer: A Transient-state Kinetics, Directed Mutagenesis, EPR and NMR Study
J. Biol. Chem., 290: 23201-23213
[ 2015 ]
Saez-Jimenez V, Fernandez-Fueyo E, Medrano FJ, Romero A, Martínez AT, Ruiz-Dueñas FJ Improving the pH-stability of Versatile Peroxidase by Comparative Structural Analysis with a Naturally-Stable Manganese Peroxidase
PlosOne, doi: 10.1371/journal.pone.0140984
[ 2015 ]
Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hällberg BM, Ludwig R, Divine C Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation
Nat. Commun., 6: 7542
year2014
Directed evolution of ligninolytic oxidoreductases: from functional expression to stabilization and beyond
García-Ruiz E, Maté D, González-Pérez D, Molina-Espeja P, Camarero S, Martínez AT, Ballesteros A, Alcalde M
In "Cascade Biocatalysis. Integrating Stereoselective and Environmentally Friendly Reactions", First Edition.
Edited by Sergio Riva and Wolf-Dieter Fessner. Wiley-VCH Verlag GmbH & Co
The ligninolytic enzymatic consortium, formed mainly by nonspecific oxidoreductases (laccases, peroxidases, and peroxide-supplying oxidases), is a potentially powerful multipurpose tool for industrial and environmental biotechnology. In nature, these enzymes are typically produced by basidiomycete white-rot fungi that are involved in lignin decay. Thanks to their broad substrate specificity, high redox potential, and minimal requirements, these enzymes have many potential applications in the field of green chemistry, including the production of biofuels, bioremediation, organic syntheses, pulp biobleaching, food and textile industries, and the design of bionanodevices. The implementation of this enzymatic armoury in different biotechnological sectors has been hampered by the lack of appropriate molecular instruments (including heterologous hosts for directed evolution) with which to improve their properties. Over the last 10 years, a wealth of directed evolution strategies in combination with hybrid approaches has emerged in order to adapt these oxidoreductases to the drastic conditions associated with many biotechnological settings (e.g., high temperatures, the presence of organic co-solvents, extreme pHs, the presence of inhibitors). This chapter summarizes all efforts and endeavors to convert these ligninolytic enzymes into useful biocatalysts by means of directed evolution: from functional expression to stabilization and beyond.
Official webpage of
[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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