Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2014 ]
Fernandez-Fueyo E, Castanera ER, Ruiz-Dueñas FJ, López-Lucendo MF, Ramírez L, Pisabarro AG, Martínez AT Ligninolytic peroxidase gene expression by Pleurotus ostreatus: Differential regulation in lignocellulose medium and effect of temperature and pH
Fungal Gen. Biol., doi: 10.1016/j.fgb.2014.02.003
[ 2014 ]
Fernandez-Fueyo E, Ruiz-Dueñas FJ, Martínez AT Engineering a fungal peroxidase that degrades lignin at very acidic pH
Biotechnol. Biofuels, 7: 114
[ 2014 ]
Fernandez-Fueyo E, Ruiz-Dueñas FJ, Martínez MJ, Romero A, Hammel KE, Medrano FJ, Martínez AT Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability
Biotechnol. Biofuels, 7: 2
[ 2014 ]
García-Ruiz E, Maté D, González-Pérez D, Molina-Espeja P, Camarero S, Martínez AT, Ballesteros A, Alcalde M Directed evolution of ligninolytic oxidoreductases: from functional expression to stabilization and beyond
In "Cascade Biocatalysis. Integrating Stereoselective and Environmentally Friendly Reactions", First Edition.
Edited by Sergio Riva and Wolf-Dieter Fessner. Wiley-VCH Verlag GmbH & Co
[ 2014 ]
González-Pérez D, Alcalde M Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae
Bioengineered, 5: 254-263
[ 2014 ]
González-Pérez D, García-Ruiz E, Ruiz-Dueñas FJ, Martínez AT, Alcalde M Structural determinants of oxidative stabilization in an evolved versatile peroxidase
ACS-Catalysis, 4: 3891-3901
year2014
Directed evolution of ligninolytic oxidoreductases: from functional expression to stabilization and beyond
García-Ruiz E, Maté D, González-Pérez D, Molina-Espeja P, Camarero S, Martínez AT, Ballesteros A, Alcalde M
In "Cascade Biocatalysis. Integrating Stereoselective and Environmentally Friendly Reactions", First Edition.
Edited by Sergio Riva and Wolf-Dieter Fessner. Wiley-VCH Verlag GmbH & Co
The ligninolytic enzymatic consortium, formed mainly by nonspecific oxidoreductases (laccases, peroxidases, and peroxide-supplying oxidases), is a potentially powerful multipurpose tool for industrial and environmental biotechnology. In nature, these enzymes are typically produced by basidiomycete white-rot fungi that are involved in lignin decay. Thanks to their broad substrate specificity, high redox potential, and minimal requirements, these enzymes have many potential applications in the field of green chemistry, including the production of biofuels, bioremediation, organic syntheses, pulp biobleaching, food and textile industries, and the design of bionanodevices. The implementation of this enzymatic armoury in different biotechnological sectors has been hampered by the lack of appropriate molecular instruments (including heterologous hosts for directed evolution) with which to improve their properties. Over the last 10 years, a wealth of directed evolution strategies in combination with hybrid approaches has emerged in order to adapt these oxidoreductases to the drastic conditions associated with many biotechnological settings (e.g., high temperatures, the presence of organic co-solvents, extreme pHs, the presence of inhibitors). This chapter summarizes all efforts and endeavors to convert these ligninolytic enzymes into useful biocatalysts by means of directed evolution: from functional expression to stabilization and beyond.
Official webpage of
[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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