Optimized oxidoreductases for medium and large scale industrial biotransformations
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Project Secretariat
Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
Phone: 34 918373112
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publications
Total records: 126
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[ 2015 ] Bormann S, Gomez Baraibar A, Ni Y, Holtmann D, Hollmann F Specific oxyfunctionalisations catalysed by peroxygenases: opportunities, challenges and solutions Catal. Sci. Technol., 5: 2038-2052
[ 2015 ] Büttner E, Ullrich R, Strittmatter E, Piontek K, Plattner D, Hofrichter M, Liers C Oxidation and nitration of mononitrophenols by a DyP-type peroxidase Arch. Biochem. Biophys., 574: 86-92
[ 2015 ] Fernandez-Fueyo E, Linde D, Almendral D, López-Lucendo MF, Ruiz-Dueñas FJ, Martínez AT Description of the first fungal dye-decolorizing peroxidase oxidizing manganese(II) Appl. Microbiol. Biotechnol., doi: 10.1007/s00253-015-6665-3
[ 2015 ] Fernandez-Fueyo E, van Wingerden M, Renirie R, Wever R, Ni Y, Holtmann D, Hollmann F Chemoenzymatic Halogenation of Phenols by using the Haloperoxidase from Curvularia inaequalis ChemCatChem, doi: 10.1002/cctc.201500862
[ 2015 ] Ferreira P, Carro J, Serrano A, Martínez AT A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes Mycologia, 107: 1105-1119
[ 2015 ] Ferreira P, Hernández-Ortega A, Lucas F, Carro J, Herguedas B, Borrelli K, Guallar V, Martínez AT, Medina M Aromatic stacking interactions govern catalysis in aryl-alcohol oxidase FEBS J., 282: 3091-3106
year2015
In-Depth 2D NMR Study of Lignin Modification During Pretreatment of Eucalyptus Wood with Laccase and Mediators
Rico A, Rencoret J, del Río JC, Martínez AT, Gutiérrez A
Bioenerg. Res., 8: 211-230

Eucalypt feedstock was pretreated with Myceliophthora thermophila laccase and methyl syringate, as mediator, in a multistage sequence consisting of successive enzymatic and alkaline peroxide stages, directly on the ground wood. Lignin modification was studied by two-dimensional (2D) nuclear magnetic resonance (NMR) of wood (at the gel state) after each stage of the sequence. Decrease of guaiacyl and syringyl lignin units (with preferential removal of the former) and aliphatic (mainly β-O-4′-linked) side chains was observed along the sequence, without a substantial change in polysaccharide cross-signals along the sequence, was observed. However, the most noticeable modification was the formation of Cα-oxidized syringyl units in the enzymatic stages, which were partially removed in the alkaline extractions. Further insight was attained by 2D NMR of the cellulolytic-enzyme lignins, isolated from the pretreated samples, and the filtrates from the different stages. Additionally, a comparison of eucalypt delignification with the high redox potential laccase from Pycnoporus cinnabarinus in the presence of the synthetic mediator 1-hydroxybenzotriazole was carried out. Wood delignification already occurred at the first cycle of the pretreatment with P. cinnabarinus laccase and 1-hydroxybenzotriazole, which directly correlated with increases in glucose (~10 %) yields after enzymatic hydrolysis. In contrast, the first cycle of pretreatment with M. thermophila laccase and methyl syringate did not produce any delignification or saccharification improvement. However, at the end of the sequence, similar delignification (~50 %) of eucalypt feedstock and increases (~30 %) in glucose yields were attained in both laccase-mediator pretreatments. Finally, the influence of lignin content and the ratio of its syringyl and guaiacyl units on recalcitrance of eucalypt feedstock to enzymatic saccharification is discussed.

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón