Optimized oxidoreductases for medium and large scale industrial biotransformations
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Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
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publications
Total records: 126
Pages:    1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21  

[ 2015 ] Hofrichter M, Kellner H, Pecyna MJ, Ullrich R Fungal unspecific peroxygenases: heme-thiolate proteins that combine peroxidase and cytochrome p450 properties Adv. Exp. Med. Biol., 851: 341-368
[ 2015 ] Kracher D, Zahma K, Schulz C, Sygmund C, Gorton L, Ludwig R Inter-domain electron transfer in cellobiose dehydrogenase: modulation by pH and divalent cations FEBS J., doi: 10.1111/febs.13310
[ 2015 ] Linde D, Pogni R, Cañellas M, Lucas F, Guallar V, Baratto MC, Sinicropi A, Saez-Jimenez V, Coscolín C, Romero A, Medrano FJ, Ruiz-Dueñas FJ, Martínez AT Catalytic surface radical in dye-decolorizing peroxidase: A computational, spectroscopic and site-directed mutagenesis study Biochem. J., 466: 253-262
[ 2015 ] Linde D, Ruiz-Dueñas FJ, Fernandez-Fueyo E, Guallar V, Hammel KE, Pogni R, Martínez AT Basidiomycete DyPs: Genomic diversity, structural-functional aspects, reaction mechanism and environmental significance Arch. Biochem. Biophys., 574: 66-74
[ 2015 ] Maté D, Alcalde M Laccase engineering: From rational design to directed evolution Biotechnol. Adv., 33: 25-40
[ 2015 ] Molina-Espeja P, Ma S, Maté D, Ludwig R, Alcalde M Tandem-yeast expression system for engineering and producing unspecific peroxygenase Enz. Microb. Technol., 73: 29-33
year2015
Laccase engineering: From rational design to directed evolution
Maté D, Alcalde M
Biotechnol. Adv., 33: 25-40

Laccases are multicopper oxidoreductases considered by many in the biotechonology field as the ultimate “green catalysts”. This is mainly due to their broad substrate specificity and relative autonomy (they use molecular oxygen from air as an electron acceptor and they only produce water as by-product), making them suitable for a wide array of applications: biofuel production, bioremediation, organic synthesis, pulp biobleaching, textiles, the beverage and food industries, biosensor and biofuel cell development. Since the beginning of the 21st century, specific features of bacterial and fungal laccases have been exhaustively adapted in order to reach the industrial demands for high catalytic activity and stability in conjunction with reduced production cost. Among the goals established for laccase engineering, heterologous functional expression, improved activity and thermostability, tolerance to non-natural media (organic solvents, ionic liquids, physiological fluids) and resistance to different types of inhibitors are all challenges that have been met, while obtaining a more comprehensive understanding of laccase structure–function relationships. In this review we examine the most significant advances in this exciting research area in which rational, semi-rational and directed evolution approaches have been employed to ultimately convert laccases into high value-added biocatalysts.

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón