Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2015 ]
Monza E, Lucas F, Camarero S, Alejaldre LC, Martínez AT, Guallar V Insights into Laccase Engineering from Molecular Simulations: Toward a Binding-Focused Strategy
J. Phys. Chem. Lett., 6: 1447-1453
[ 2015 ]
Ni Y, Fernandez-Fueyo E, Baraibar AG, Ullrich R, Hofrichter M, Yanase H, Alcalde M, van Berkel WJ, Hollmann F Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol
Angew. Chem. Int. Ed., doi: 10.1002/anie.201507881
[ 2015 ]
Pardo I, Camarero S Laccase engineering by rational and evolutionary design
Cell Mol. Life Sci., doi: 10.1007/s00018-014-1824-8
[ 2015 ]
Pardo I, Camarero S Exploring the Oxidation of Lignin-Derived Phenols by a Library of Laccase Mutants
Molecules, 20: 15929-15943
[ 2015 ]
Pezzella C, Guarino L, Piscitelli A How to enjoy laccases
Cell Mol. Life Sci., 72: 923-940
[ 2015 ]
Pham NH, Hollmann F, Kracher D, Preims M, Haltrich D, Ludwig R Engineering an enzymatic regeneration system for NAD(P)H oxidation
J. Mol. Cat. B, 120: 38-46
year2015
Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol
Ni Y, Fernandez-Fueyo E, Baraibar AG, Ullrich R, Hofrichter M, Yanase H, Alcalde M, van Berkel WJ, Hollmann F
Angew. Chem. Int. Ed., doi: 10.1002/anie.201507881
Peroxygenases catalyze a broad range of (stereo)selective oxyfunctionalization reactions. However, to access their full catalytic potential, peroxygenases need a balanced provision of hydrogen peroxide to achieve high catalytic activity while minimizing oxidative inactivation. Herein, we report an enzymatic cascade process that employs methanol as a sacrificial electron donor for the reductive activation of molecular oxygen. Full oxidation of methanol is achieved, generating three equivalents of hydrogen peroxide that can be used completely for the stereoselective hydroxylation of ethylbenzene as a model reaction. Overall we propose and demonstrate an atom-efficient and easily applicable alternative to established hydrogen peroxide generation methods, which enables the efficient use of peroxygenases for oxyfunctionalization reactions.
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[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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