Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2016 ]
Pardo I, Santiago G, Gentili P, Lucas F, Monza E, Medrano FJ, Galli C, Martínez AT, Guallar V, Camarero S Re-designing the substrate binding pocket of laccase for enhanced oxidation of sinapic acid
Catal. Sci. Technol., doi: 10.1039/C5CY01725D
[ 2016 ]
Rencoret J, Pereira A, del Río JC, Martínez AT, Gutiérrez A Laccase-Mediator Pretreatment of Wheat Straw Degrades Lignin and Improves Saccharification
Bioenerg. Res., 9: 917-930
[ 2016 ]
Saez-Jimenez V, Acebes S, García-Ruiz E, Romero A, Guallar V, Alcalde M, Medrano FJ, Martínez AT, Ruiz-Dueñas FJ Unveiling the basis of alkaline stability of an evolved versatile peroxidase
Biochem. J., 473: 1917-1928
[ 2016 ]
Saez-Jimenez V, Rencoret J, Rodríguez-Carvajal MA, Gutiérrez A, Ruiz-Dueñas FJ, Martínez AT Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin
Biotechnol. Biofuels, 9: 198-211
[ 2016 ]
Salvachúa D, Katahira R, Cleveland NS, Khanna P, Resch MG, Black BA, Purvine SO, Zink EM, Prieto A, Martínez MJ, Martínez AT, Simmons BA, Gladden JM, Beckham GT Lignin depolymerization by fungal secretomes and a microbial sink
Green Chem., doi: 10.1039/C6GC01531J
[ 2016 ]
Santiago G, de Salas F, Lucas F, Monza E, Acebes S, Martínez AT, Camarero S, Guallar V Computer-Aided Laccase Engineering: Toward Biological Oxidation of Arylamines
ACS-Catalysis, 6: 5415-5423
year2016
Computer-Aided Laccase Engineering: Toward Biological Oxidation of Arylamines
Santiago G, de Salas F, Lucas F, Monza E, Acebes S, Martínez AT, Camarero S, Guallar V
ACS-Catalysis, 6: 5415-5423
Oxidation of arylamines, such as aniline, is of high industrial interest, and laccases have been proposed as biocatalysts to replace harsh chemical oxidants. However, the reaction is hampered by the redox potential of the substrate at acid pH, and enzyme engineering is required to improve the oxidation. In this work, instead of trying to improve the redox potential of the enzyme, we aim toward the (transient) substrate’s potential and propose this as a more reliable strategy. We have successfully combined a computational approach with experimental validation to rationally design an improved biocatalyst. The in silico protocol combines classical and quantum mechanics to deliver atomic and electronic level detail on the two main processes involved: substrate binding and electron transfer. After mutant expression and comparison to the parent type, kinetic results show that the protocol accurately predicts aniline’s improved oxidation (2-fold kcat increase) in the engineered variant for biocatalyzed polyaniline production.
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[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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