Optimized oxidoreductases for medium and large scale industrial biotransformations
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Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
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publications
Total records: 126
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[ 2015 ] Monza E, Lucas F, Camarero S, Alejaldre LC, Martínez AT, Guallar V Insights into Laccase Engineering from Molecular Simulations: Toward a Binding-Focused Strategy J. Phys. Chem. Lett., 6: 1447-1453
[ 2015 ] Ni Y, Fernandez-Fueyo E, Baraibar AG, Ullrich R, Hofrichter M, Yanase H, Alcalde M, van Berkel WJ, Hollmann F Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol Angew. Chem. Int. Ed., doi: 10.1002/anie.201507881
[ 2015 ] Pardo I, Camarero S Laccase engineering by rational and evolutionary design Cell Mol. Life Sci., doi: 10.1007/s00018-014-1824-8
[ 2015 ] Pardo I, Camarero S Exploring the Oxidation of Lignin-Derived Phenols by a Library of Laccase Mutants Molecules, 20: 15929-15943
[ 2015 ] Pezzella C, Guarino L, Piscitelli A How to enjoy laccases Cell Mol. Life Sci., 72: 923-940
[ 2015 ] Pham NH, Hollmann F, Kracher D, Preims M, Haltrich D, Ludwig R Engineering an enzymatic regeneration system for NAD(P)H oxidation J. Mol. Cat. B, 120: 38-46
year2019
Different fungal peroxidases oxidize nitrophenols at a surface catalytic tryptophan
Linde D, Ayuso-Fernández I, Ruiz-Dueñas FJ, Martínez AT
Arch. Biochem. Biophys., 668: 23-28

Dye-decolorizing peroxidase (DyP) from Auricularia auricula-judae and versatile peroxidase (VP) from Pleurotus eryngii oxidize the three mononitrophenol isomers. Both enzymes have been overexpressed in Escherichia coli and in vitro activated. Despite their very different three-dimensional structures, the nitrophenol oxidation site is located at a solvent-exposed aromatic residue in both DyP (Trp377) and VP (Trp164), as revealed by liquid chromatography coupled to mass spectrometry and kinetic analyses of nitrophenol oxidation by the native enzymes and their tryptophan-less variants (the latter showing 10–60 fold lower catalytic efficiencies).

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Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón