Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2015 ]
Viña-Gonzalez J, González-Pérez D, Ferreira P, Martínez AT, Alcalde M Focused directed evolution of aryl-alcohol oxidase in yeast using chimeric signal peptides
Appl. Environ. Microbiol., 81: 6451-6462
[ 2015 ]
Wang X, Ullrich R, Hofrichter M, Groves JT Heme-thiolate ferryl of aromatic peroxygenase is basic and reactive
Proc. Natl. Acad. Sci. USA, 112: 3686-3691
[ 2014 ]
Barrasa JM, Blanco MN, Esteve-Raventós F, Altés A, Checa J, Martínez AT, Ruiz-Dueñas FJ Wood and humus decay strategies by white-rot basidiomycetes correlate with two different dye decolorization and enzyme secretion patterns on agar plates
Fungal Gen. Biol., doi: 10.1016/j.fgb.2014.03.007
[ 2014 ]
Camarero S, Martínez MJ, Martínez AT Understanding lignin biodegradation for the improved utilization of plant biomass in modern biorefineries
Biofuels, Bioprod. Bioref., 8: 615-625
[ 2014 ]
Carro J, Ferreira P, Rodríguez L, Prieto A, Serrano A, Balcells B, Ardá A, Jiménez-Barbero J, Gutiérrez A, Ullrich R, Hofrichter M, Martínez AT 5-Hydroxymethylfurfural conversion by fungal aryl-alcohol oxidase and unspecific peroxygenase
FEBS J., 282: 3218-3229
[ 2014 ]
Fernandez-Fueyo E, Acebes S, Ruiz-Dueñas FJ, Martínez MJ, Romero A, Medrano FJ, Guallar V, Martínez AT Structural implications of the C-terminal tail in the catalytic and stability properties of manganese peroxidases from ligninolytic fungi
Acta Crystal. D, 70: 3253-3265
year2014
Mutagenic Organized Recombination Process by Homologous In vivo Grouping (MORPHING) for directed enzyme evolution
González-Pérez D, Molina-Espeja P, García-Ruiz E, Alcalde M
PlosOne, 9: 3
Approaches that depend on directed evolution require reliable methods to generate DNA diversity so that mutant libraries can focus on specific target regions. We took advantage of the high frequency of homologous DNA recombination in Saccharomyces cerevisiae to develop a strategy for domain mutagenesis aimed at introducing and in vivo recombining random mutations in defined stretches of DNA. Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) is a one-pot random mutagenic method for short protein segments that harnesses the in vivo recombination apparatus of yeast. Using this approach, libraries can be prepared with different mutational loads in DNA stretches of less than 30 amino acids, and these DNA stretches can be assembled into the remaining unaltered DNA regions in vivo with high fidelity. The combination of MORPHING with classical directed evolution and semi-rational approaches, or with neutral genetic drift, may lead to the development of new adaptive pathways to engineer more robust biocatalysts.
Official webpage of
[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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