Optimized oxidoreductases for medium and large scale industrial biotransformations
Total records:
126
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[ 2016 ]
Maté D, Alcalde M Laccase: a multi-purpose biocatalyst at the forefront of biotechnology
Microbial Biotechnol., doi: 10.1111/1751-7915.12422
[ 2016 ]
Mathieu Y, Piumi F, Valli R, Carro J, Ferreira P, Faulds CB, Record E Activities of Secreted Aryl Alcohol Quinone Oxidoreductases from Pycnoporus cinnabarinus Provide Insights into Fungal Degradation of Plant Biomass
Appl. Environ. Microbiol., 82: 2411-2423
[ 2016 ]
Molina-Espeja P, Cañellas M, Plou FJ, Hofrichter M, Lucas F, Guallar V, Alcalde M Synthesis of 1-Naphthol by a Natural Peroxygenase engineered by Directed Evolution
ChemBioChem, 17: 341-349
[ 2016 ]
Molina-Espeja P, Viña-Gonzalez J, Gomez-Fernandez BJ, Martin-Diaz J, García-Ruiz E, Alcalde M Beyond the outer limits of nature by directed evolution
Biotechnol. Adv., 34: 754-767
[ 2016 ]
Ni Y, Fernandez-Fueyo E, Gomez Baraibar A, Ullrich R, Hofrichter M, Yanase H, Alcalde M, van Berkel WJ, Hollmann F Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol
Angew. Chem. Int. Ed., 55: 798-801
[ 2016 ]
Olmedo A, Aranda C, del Río JC, Kiebist J, Scheibner K, Martínez AT, Gutiérrez A From Alkanes to Carboxylic Acids: Terminal Oxygenation by a Fungal Peroxygenase
Angew. Chem. Int. Ed., 55: 12248-12251
year2016
Computer-Aided Laccase Engineering: Toward Biological Oxidation of Arylamines
Santiago G, de Salas F, Lucas F, Monza E, Acebes S, Martínez AT, Camarero S, Guallar V
ACS-Catalysis, 6: 5415-5423
Oxidation of arylamines, such as aniline, is of high industrial interest, and laccases have been proposed as biocatalysts to replace harsh chemical oxidants. However, the reaction is hampered by the redox potential of the substrate at acid pH, and enzyme engineering is required to improve the oxidation. In this work, instead of trying to improve the redox potential of the enzyme, we aim toward the (transient) substrate’s potential and propose this as a more reliable strategy. We have successfully combined a computational approach with experimental validation to rationally design an improved biocatalyst. The in silico protocol combines classical and quantum mechanics to deliver atomic and electronic level detail on the two main processes involved: substrate binding and electron transfer. After mutant expression and comparison to the parent type, kinetic results show that the protocol accurately predicts aniline’s improved oxidation (2-fold kcat increase) in the engineered variant for biocatalyzed polyaniline production.
Official webpage of
[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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